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SLC25A46 Recombinant antibody, PBS Only (Capture)

SLC25A46 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240114G3

Cat no : 83360-2-PBS

Synonyms

TB1, Mitochondrial outer membrane protein SLC25A46, 240114G3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A46 Recombinant antibody (83360-2-RR)

    WB analysis using 83360-2-RR (same clone as 83360-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83360-2-RR (SLC25A46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83360-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Caco-2 cells using SLC25A46 Recombinant antibody (83360-2-RR)

    FC experiment of Caco-2 using 83360-2-RR (same clone as 83360-2-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83360-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SLC25A46 Recombinant antibody (83360-2-RR)

    FC experiment of HeLa using 83360-2-RR (same clone as 83360-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC25A46 Recombinant antibody (83360-2-RR, Clone:240114G3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83360-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00369-1

    Cytometric bead array standard curve of MP00369-1

    Cytometric bead array standard curve of MP00369-1, SLC25A46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83360-2-PBS. Detection antibody: 83360-3-PBS. Standard: Ag27293. Range: 0.625-80ng/mL.

  • Affinity and Kinetic Characterization of 83360-2-RR

    Affinity and Kinetic Characterization of 83360-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83360-2-RR against Human SLC25A46 were performed. The affinity constant is 0.879 nM.

Product Information

83360-2-PBS targets SLC25A46 as part of a matched antibody pair:

MP00369-1: 83360-2-PBS capture and 83360-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SLC25A46 fusion protein Ag27293
Full Name solute carrier family 25, member 46
Calculated Molecular Weight 418 aa, 46 kDa
GenBank Accession NumberBC017169
Gene Symbol SLC25A46
Gene ID (NCBI) 91137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.