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NRG1, isoform Alpha Recombinant antibody, PBS Only (Detector)

NRG1, isoform Alpha Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240067A6

Cat no : 83251-2-PBS

Synonyms

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NRG1, isoform Alpha Recombinant antibody (83251-2-RR)

    IF Staining of MCF-7 using 83251-2-RR (same clone as 83251-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83251-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00232-1

    Cytometric bead array standard curve of MP00232-1

    Cytometric bead array standard curve of MP00232-1, NRG1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83251-3-PBS. Detection antibody: 83251-2-PBS. Standard: Ag35114. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83251-2-RR

    Affinity and Kinetic Characterization of 83251-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83251-2-RR against Human USP30 were performed. The affinity constant is 11.9 nM.

Product Information

83251-2-PBS targets NRG1, isoform Alpha as part of a matched antibody pair:

MP00232-1: 83251-3-PBS capture and 83251-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NRG1, isoform Alpha fusion protein Ag35114
Full Name neuregulin 1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007675
Gene Symbol NRG1
Gene ID (NCBI) 3084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.