NRG1, isoform Alpha Recombinant antibody

• 

  IF Staining of rat brain using 83251-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 83251-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NRG1, isoform Alpha antibody (83251-2-RR, Clone: 240067A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NRG1, isoform Alpha Uni-rAb^TM Recombinant Antibody for IF/ICC, IF-P, ELISA

Cat No. 83251-2-RR

Clone No.240067A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P, ELISA

Synonyms

NRG1, NRG1A, Heregulin, Glial growth factor, GGF2

---

Tested Applications

• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83251-2-RR targets NRG1, isoform Alpha in IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NRG1, isoform Alpha fusion protein Ag35114
• Full Name: neuregulin 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007675
• Gene Symbol: NRG1
• Gene ID (NCBI): 3084
• RRID: AB_3670926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02297
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. The immunogen of this antibody is against NRG1 isoform Alpha.