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SOX8 Recombinant antibody

SOX8 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240120H6

Cat no : 83238-3-RR

Synonyms

Transcription factor SOX-8, Transcription factor SOX 8, 240120H6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOX8 Recombinant antibody (83238-3-RR)

    IF Staining of HeLa using 83238-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83238-3-RR

    Affinity and Kinetic Characterization of 83238-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83238-3-RR against Human SOX8 were performed. The affinity constant is 1.98 nM.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83238-3-RR targets SOX8 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SOX8 fusion protein Ag34395
Full Name SRY (sex determining region Y)-box 8
Calculated Molecular Weight 446 aa, 47 kDa
GenBank Accession NumberBC031797
Gene Symbol SOX8
Gene ID (NCBI) 30812
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SOX8 antibody 83238-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols