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SULT1B1 Recombinant antibody, PBS Only (Capture)

SULT1B1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240119C12

Cat no : 83228-1-PBS

Synonyms

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1, 240119C12

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using SULT1B1 Recombinant antibody (83228-1-RR)

    WB analysis of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 83228-1-RR (SULT1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SULT1B1 Recombinant antibody (83228-1-RR)

    IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SULT1B1 Recombinant antibody (83228-1-RR)

    IHC staining of mouse colon using 83228-1-RR (same clone as 83228-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using SULT1B1 Recombinant antibody (83228-1-RR)

    FC experiment of A431 using 83228-1-RR (same clone as 83228-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SULT1B1 Recombinant antibody (83228-1-RR, Clone:240119C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83228-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00184-1

    Cytometric bead array standard curve of MP00184-1

    Cytometric bead array standard curve of MP00184-1, SULT1B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83228-1-PBS. Detection antibody: 83228-2-PBS. Standard: Ag9009. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83228-1-RR

    Affinity and Kinetic Characterization of 83228-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83228-1-RR against Human SULT1B1 were performed. The affinity constant is 63.8 nM.

Product Information

83228-1-PBS targets SULT1B1 as part of a matched antibody pair:

MP00184-1: 83228-1-PBS capture and 83228-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SULT1B1 fusion protein Ag9009
Full Name sulfotransferase family, cytosolic, 1B, member 1
Calculated Molecular Weight 296 aa, 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC010895
Gene Symbol SULT1B1
Gene ID (NCBI) 27284
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).