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NUDCD2 Recombinant antibody

NUDCD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230513A5

Cat no : 83217-5-RR

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDCD2 Recombinant antibody (83217-5-RR)

    WB analysis using 83217-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    IP experiment of HepG2 using 83217-5-RR

    IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    IF Staining of HepG2 using 83217-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    FC experiment of HepG2 using 83217-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83217-5-RR

    Affinity and Kinetic Characterization of 83217-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, U-251 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83217-5-RR is NUDCD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUDCD2 fusion protein Ag15579
Full Name NudC domain containing 2
Calculated Molecular Weight 157 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC017934
Gene Symbol NUDCD2
Gene ID (NCBI) 134492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

Protocols

Product Specific Protocols
WB protocol for NUDCD2 antibody 83217-5-RRDownload protocol
IF protocol for NUDCD2 antibody 83217-5-RRDownload protocol
IP protocol for NUDCD2 antibody 83217-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols