Filter: AllWBIPIF/ICCFC (Intra)Affinity

WB analysis of HEK-293 using 83217-5-RR
WB result of NUDCD2 antibody (83217-5-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.
WB analysis using 83217-5-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 83217-5-RR
IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug.
IF Staining of HepG2 using 83217-5-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HepG2 using 83217-5-RR
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 83217-5-RR
Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

Featured Product
KD/KO Validated

NUDCD2 Recombinant antibody

NUDCD2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Cat No. 83217-5-RR

Clone No.230513A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, HepG2 cells, U-251 cells
Positive IP detected in	HepG2 cells
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83217-5-RR targets NUDCD2 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	NUDCD2 fusion protein Ag15579 Predict reactive species
Full Name	NudC domain containing 2
Calculated Molecular Weight	157 aa, 18 kDa
Observed Molecular Weight	18 kDa
GenBank Accession Number	BC017934
Gene Symbol	NUDCD2
Gene ID (NCBI)	134492
RRID	AB_3670903
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q8WVJ2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

Protocols

Product Specific Protocols
WB protocol for NUDCD2 antibody 83217-5-RR	Download protocol
IF protocol for NUDCD2 antibody 83217-5-RR	Download protocol
IP protocol for NUDCD2 antibody 83217-5-RR	Download protocol