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  • Western Blot (WB) analysis of various lysates using CEACAM6 Recombinant antibody (83203-5-RR)

    WB analysis using 83203-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83203-5-RR (CEACAM6 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83203-5-RR

    Affinity and Kinetic Characterization of 83203-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83203-5-RR against Human CEACAM6 were performed. The affinity constant is 0.113 nM.

CEACAM6 Recombinant antibody

CEACAM6 {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 83203-5-RR
Clone No.230475D5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Non-specific crossreacting antigen, NCA, CEAL, CD66c, Carcinoembryonic antigen-related cell adhesion molecule 6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83203-5-RR targets CEACAM6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein Predict reactive species
Full Name carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight40-100 kDa
GenBank Accession NumberBC005008
Gene Symbol CEACAM6
Gene ID (NCBI) 4680
RRIDAB_3670891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40199
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEACAM6, also known as CD66c, is a glycosylphosphatidylinositol (GPI)-linked cell surface protein that belongs to the CEACAM family of the immunoglobulin superfamily (PMID: 23903773). It is normally expressed on the surface of myeloid and epithelial surfaces and upregulated preferentially at the apical/luminal membranes of many tumors (PMID: 27595061; 35082925). CEACAM6 plays an important role in cell adhesion, proliferation, tumorigenesis, metastasis, and immunity (PMID: 31797958).

Protocols

Product Specific Protocols
WB protocol for CEACAM6 antibody 83203-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols