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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIRA Recombinant antibody (83194-5-RR)

    IF Staining of HeLa using 83194-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HIRA Recombinant antibody (83194-5-RR)

    IF Staining of HeLa using 83194-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K-562 cells using HIRA Recombinant antibody (83194-5-RR)

    FC experiment of K-562 using 83194-5-RR

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant Antibody (83194-5-RR, Clone:240006E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83194-5-RR

    Affinity and Kinetic Characterization of 83194-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83194-5-RR against Human HIRA were performed. The affinity constant is 7.91 nM.

HIRA Recombinant antibody

HIRA {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83194-5-RR
Clone No.240006E5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Protein HIRA, HIRA/HIR, HIR, 240006E5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83194-5-RR targets HIRA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIRA fusion protein Ag34439 Predict reactive species
Full Name HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
Calculated Molecular Weight 112 kDa
GenBank Accession NumberNM_003325
Gene Symbol HIRA
Gene ID (NCBI) 7290
RRIDAB_3670884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP54198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIRA protein is encoded by a gene within a region of human chromosome 22q11.2 deleted in most patients with the DiGeorge syndrome, a developmental disorder, and was named for its amino acid sequence homology to two S. cerevisiae proteins, Hir1p and Hir2p. HIRA is critical in a specific chromatin assembly pathway that takes place independently of DNA synthesis (PMID: 12049744). HIRA complex associates with various proteins, such as transcription factors, RNA pol II, Prohibitin (PHB), and replication protein A (RPA) suggests that the targeting of the complex at specific locations may exploit additional structural properties and mechanisms (PMID: 30082790).

Protocols

Product Specific Protocols
IF protocol for HIRA antibody 83194-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols