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SAR1A Recombinant antibody

SAR1A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230471A11

Cat no : 83168-6-RR

Synonyms

SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SAR1A Recombinant antibody (83168-6-RR)

    WB analysis using 83168-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83168-6-RR (SAR1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using SAR1A Recombinant antibody (83168-6-RR)

    FC experiment of A549 using 83168-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-6-RR, Clone:230471A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A431 cells using SAR1A Recombinant antibody (83168-6-RR)

    FC experiment of A431 using 83168-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-6-RR, Clone:230471A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293T cells, NIH/3T3 cells, HeLa cells, Jurkat cells, mouse liver tissue
Positive FC (Intra) detected inA549 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83168-6-RR targets SAR1A in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name SAR1 homolog A (S. cerevisiae)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight23-25 kDa
GenBank Accession NumberNM_020150
Gene Symbol SAR1A
Gene ID (NCBI) 56681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).

Protocols

Product Specific Protocols
WB protocol for SAR1A antibody 83168-6-RRDownload protocol
FC protocol for SAR1A antibody 83168-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols