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PCYT1A Recombinant antibody

PCYT1A Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230393G2

Cat no : 83100-2-RR

Synonyms

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PCYT1A Recombinant antibody (83100-2-RR)

    IF Staining of MCF-7 using 83100-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PCYT1A antibody (83100-2-RR, Clone: 230393G2 ) at dilution of 1:25 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using PCYT1A Recombinant antibody (83100-2-RR)

    FC experiment of A431 using 83100-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-2-RR, Clone:230393G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83100-2-RR targets PCYT1A in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCYT1A fusion protein Ag34268
Full Name phosphate cytidylyltransferase 1, choline, alpha
Calculated Molecular Weight42 kDa
GenBank Accession NumberNM_005017
Gene Symbol PCYT1A
Gene ID (NCBI) 5130
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for PCYT1A antibody 83100-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols