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  • Western Blot (WB) analysis of various lysates using STK39 Recombinant antibody (83081-2-RR)

    WB analysis using 83081-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83081-2-RR (STK39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STK39 Recombinant antibody (83081-2-RR)

    IF Staining of mouse brain using 83081-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using STK39 Recombinant antibody (83081-2-RR)

    IF Staining of NIH/3T3 using 83081-2-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83081-2-RR

    Affinity and Kinetic Characterization of 83081-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83081-2-RR against Human STK39 were performed. The affinity constant is 2.52 nM.

STK39 Recombinant antibody

STK39 Recombinant Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 83081-2-RR
Clone No.230382G9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, SH-SY5Y cells, HEK-293T cells, HepG2 cells, rat brain tissue
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83081-2-RR targets STK39 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STK39 fusion protein Ag34503 Predict reactive species
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Calculated Molecular Weight59 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
RRIDAB_3670797
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).

Protocols

Product Specific Protocols
WB protocol for STK39 antibody 83081-2-RRDownload protocol
IF protocol for STK39 antibody 83081-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols