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ECM1 Recombinant antibody, PBS Only (Capture/Detector)

ECM1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230321A4

Cat no : 83067-1-PBS

Synonyms

Secretory component p85, extracellular matrix protein 1, 230321A4

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00011-4

    Sandwich ELISA standard curve of MP00011-4

    Sandwich ELISA standard curve of MP00011-4, Human ECM1 Recombinant Matched Antibody Pair - PBS only. 83067-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0533. 83067-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00011-4

    Sample test of MP00011-4

    Plasma of fourteen individual healthy human donors was measured. The ECM1 concentration of detected samples was determined to be 3,683.47 ng/mL with a range of 1,495.22-6,198.62 ng/mL.

  • Sample test of MP00011-4

    Sample test of MP00011-4

    MDA-MB-468 were cultured in DMEM  supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. The cell culture supernatant was removed, assayed for human ECM1, and measured 11.6 ng/mL.

  • Sample test of MP00011-4

    Sample test of MP00011-4

    Serum of fourteen individual healthy human donors was measured. The ECM1 concentration of detected samples was determined to be 3,875.31 ng/mL with a range of 1,605.39-6,867.83 ng/mL.

  • Cytometric bead array standard curve of MP00011-3

    Cytometric bead array standard curve of MP00011-3

    Cytometric bead array standard curve of MP00011-3, ECM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83067-1-PBS. Detection antibody: 83067-4-PBS. Standard: Eg0533. Range: 0.625-20 ng/mL.

Product Information

83067-1-PBS targets ECM1 as part of a matched antibody pair:

MP00011-3: 83067-1-PBS capture and 83067-4-PBS detection (validated in Cytometric bead array)

MP00011-4: 83067-7-PBS capture and 83067-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name extracellular matrix protein 1
Calculated Molecular Weight 540 aa, 61 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.