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FDFT1 Recombinant antibody

FDFT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230362C10

Cat no : 83020-1-RR

Synonyms

Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FDFT1 Recombinant antibody (83020-1-RR)

    WB analysis using 83020-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83020-1-RR (FDFT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FDFT1 Recombinant antibody (83020-1-RR)

    IHC staining of human ovary cancer using 83020-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83020-1-RR (FDFT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FDFT1 Recombinant antibody (83020-1-RR)

    IF Staining of HepG2 using 83020-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using FDFT1 Recombinant antibody (83020-1-RR)

    IF Staining of L02 using 83020-1-RR

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of U2OS cells using FDFT1 Recombinant antibody (83020-1-RR)

    FC experiment of U2OS using 83020-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human FDFT1 (83020-1-RR, Clone:230362C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83020-1-RR

    Affinity and Kinetic Characterization of 83020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83020-1-RR against Human FDFT1 were performed. The affinity constant is 0.126 nM.

Tested Applications

Positive WB detected inL02 cells, T-47D cells, SH-SY5Y cells, HepG2 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells, HepG2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83020-1-RR targets FDFT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDFT1 fusion protein Ag3743
Full Name farnesyl-diphosphate farnesyltransferase 1
Calculated Molecular Weight 417 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC029641
Gene Symbol FDFT1
Gene ID (NCBI) 2222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).

Protocols

Product Specific Protocols
WB protocol for FDFT1 antibody 83020-1-RRDownload protocol
IHC protocol for FDFT1 antibody 83020-1-RRDownload protocol
IF protocol for FDFT1 antibody 83020-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols