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AIFM2/ FSP1 Recombinant antibody

AIFM2/ FSP1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2J1

Cat no : 82974-1-RR

Synonyms

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    WB analysis using 82974-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82974-1-RR (AIFM2/ FSP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    IP experiment of HepG2 using 82974-1-RR

    IP result of anti-AIFM2/ FSP1 (IP:82974-1-RR, 4ug; Detection:82974-1-RR 1:3000) with HepG2 cells lysate 1085 ug.

  • Flow cytometry (FC) experiment of A549 cells using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    FC experiment of A549 using 82974-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug AIFM2/ FSP1 Recombinant antibody (82974-1-RR, Clone:2J1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82974-1-RR

    Affinity and Kinetic Characterization of 82974-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82974-1-RR against Human AIFM2/ FSP1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82974-1-RR targets AIFM2/ FSP1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag13412
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC023601
Gene Symbol AIFM2/ FSP1
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Protocols

Product Specific Protocols
WB protocol for AIFM2/ FSP1 antibody 82974-1-RRDownload protocol
IP protocol for AIFM2/ FSP1 antibody 82974-1-RRDownload protocol
FC protocol for AIFM2/ FSP1 antibody 82974-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols