• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HMGB1 Recombinant antibody

HMGB1 Recombinant Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230182G6

Cat no : 82973-1-RR

Synonyms

DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using HMGB1 Recombinant antibody (82973-1-RR)

    WB analysis using 82973-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82973-1-RR (HMGB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HMGB1 Recombinant antibody (82973-1-RR)

    IHC staining of mouse brain using 82973-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HMGB1 Recombinant antibody (82973-1-RR)

    IHC staining of mouse brain using 82973-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82973-1-RR (HMGB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HMGB1 Recombinant antibody (82973-1-RR)

    IF Staining of HeLa using 82973-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HMGB1 antibody (82973-1-RR, Clone: 230182G6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). CoraLite®594-Phalloidin (red).

Tested Applications

Positive WB detected inL02 cells, Jurkat cells, HEK-293 cells, K-562 cells, HCT 116 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82973-1-RR targets HMGB1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGB1 fusion protein Ag1264
Full Name high-mobility group box 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC003378
Gene Symbol HMGB1
Gene ID (NCBI) 3146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HMGB1 antibody 82973-1-RRDownload protocol
IHC protocol for HMGB1 antibody 82973-1-RRDownload protocol
IF protocol for HMGB1 antibody 82973-1-RRDownload protocol
FC protocol for HMGB1 antibody 82973-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols