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SCN3B Recombinant antibody

SCN3B Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230316B2

Cat no : 82959-7-RR

Synonyms

HSA243396, KIAA1158, SCN3B, SCNB3, Sodium channel subunit beta 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCN3B Recombinant antibody (82959-7-RR)

    WB analysis using 82959-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82959-7-RR (SCN3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82959-7-RR

    Affinity and Kinetic Characterization of 82959-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82959-7-RR against Human SCN3B were performed. The affinity constant is 0.98 nM.

Tested Applications

Positive WB detected inHEK-293 cells, rat brain tissue, mouse brain tissue, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82959-7-RR is SCN3B Fusion Protein expressed in E. coli.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SCN3B fusion protein Ag23201
Full Name sodium channel, voltage-gated, type III, beta
Observed Molecular Weight28 kDa
GenBank Accession NumberBC126265
Gene Symbol SCN3B
Gene ID (NCBI) 55800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The sodium channel is a multi-subunit protein complex composed of a single large α-subunit along with smaller additional β-subunits. There are at least three different β-subunit genes, SCN1b, SCN2b, and SCN3b, all of which are expressed widely in excitable tissues. The bands on Western blots for SCN3b (45-50 kDa) were significantly larger than the predicted molecular weight, which is consistent with the protein being glycosylated. The two bands on the Western blot could be due to different levels of glycosylation or alternative spliced isoforms. (PMID: 11744748)

Protocols

Product Specific Protocols
WB protocol for SCN3B antibody 82959-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols