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S100A16 Recombinant antibody

S100A16 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3E1

Cat no : 82923-1-RR

Synonyms

Protein S100-A16, Protein S100 F, Protein S100 A16, Aging-associated gene 13 protein, AAG13

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of A431 cells using S100A16 Recombinant antibody (82923-1-RR)

    WB analysis of A431 using 82923-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 82923-1-RR (S100A16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using S100A16 Recombinant antibody (82923-1-RR)

    FC experiment of A431 using 82923-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A16 Recombinant antibody (82923-1-RR, Clone:3E1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82923-1-RR targets S100A16 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100A16 fusion protein Ag2023
Full Name S100 calcium binding protein A16
Calculated Molecular Weight 103 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC019099
Gene Symbol S100A16
Gene ID (NCBI) 140576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A16 is a member of the S100 protein family. S100A16 is expressed in a variety of human tissues. S100A16 expression is especially high in tissues rich in epithelial cells. Functionally, S100A16 has been linked to several aspects of tumorigenesis, for example, cell proliferation, differentiation, migration, invasion, and epithelial-mesenchymal transition (EMT).Accordingly, S100A16 has been suggested to have both tumour-promoting and suppressive roles in human cancers. S100A16-mediated cellular functions are suggested to be mediated by the regulation of various signaling pathways/proteins including EMT-related proteins E-cadherin and Vimentin, PI3K-AKT, p53, MMP1-1, MMP-2, MMP-9, JNK/p38, etc. (PMID: 37509106).

Protocols

Product Specific Protocols
WB protocol for S100A16 antibody 82923-1-RRDownload protocol
FC protocol for S100A16 antibody 82923-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols