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KPNA4 Recombinant antibody

KPNA4 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230098H1

Cat no : 82898-2-RR

Synonyms

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KPNA4 Recombinant antibody (82898-2-RR)

    WB analysis using 82898-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82898-2-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using KPNA4 Recombinant antibody (82898-2-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 82898-2-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82898-2-RR (KPNA4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using KPNA4 Recombinant antibody (82898-2-RR)

    IF Staining of U2OS using 82898-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-2-RR, Clone: 230098H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A549 cells using KPNA4 Recombinant antibody (82898-2-RR)

    FC experiment of A549 using 82898-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug KPNA4 Recombinant antibody (82898-2-RR, Clone:230098H1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82898-2-RR

    Affinity and Kinetic Characterization of 82898-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82898-2-RR against Human KPNA4 were performed. The affinity constant is 0.678 nM.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, NIH/3T3 cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82898-2-RR targets KPNA4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KPNA4 fusion protein Ag3133
Full Name karyopherin alpha 4 (importin alpha 3)
Calculated Molecular Weight 521 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC034493
Gene Symbol KPNA4
Gene ID (NCBI) 3840
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KPNA4, also named importin subunit alpha-3, karyopherin-alpha4, is a cytoplasmic protein that recognizes nuclear localization signals (NLSs) and dock NLS-containing proteins to the nuclear pore complex. Nuclear import, mediated in part by karyopherin-α (KPNA)/importin-α subtypes, regulates transcription factor access to the genome and determines cell fate. KPNA4-mediated nuclear transport of Ras-responsive element-binding protein (RREB1), which sustains Ras/ERK pathway signaling through repressing miR-143/145 expression (PMID: 31822798). KPNA4 is one of the main isoforms that is activated in many human cancers. KPNA4 expression was elevated in head and neck of squamous cell carcinoma (PMID: 33188837).

Protocols

Product Specific Protocols
WB protocol for KPNA4 antibody 82898-2-RRDownload protocol
IHC protocol for KPNA4 antibody 82898-2-RRDownload protocol
IF protocol for KPNA4 antibody 82898-2-RRDownload protocol
FC protocol for KPNA4 antibody 82898-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols