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DNMT1 Recombinant antibody

DNMT1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240302E2

Cat no : 82678-2-RR

Synonyms

AIM, CXXC9, DNA methyltransferase HsaI, DNA MTase HsaI, DNMT, DNMT1, FLJ16293, M.HsaI, MCMT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNMT1 Recombinant antibody (82678-2-RR)

    WB analysis using 82678-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82678-2-RR (DNMT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, HEK-293T cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82678-2-RR is DNMT1 Fusion Protein expressed in E. coli.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNMT1 fusion protein Ag29479
Full Name DNA (cytosine-5-)-methyltransferase 1
Calculated Molecular Weight 1632 aa, 185 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberBC126227
Gene Symbol DNMT1
Gene ID (NCBI) 1786
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation, and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. (PMID: 10753866)

Protocols

Product Specific Protocols
WB protocol for DNMT1 antibody 82678-2-RRDownload protocol
FC protocol for DNMT1 antibody 82678-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols