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Bcl2 Recombinant antibody

Bcl2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240571H5

Cat no : 82469-6-RR

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using Bcl2 Recombinant antibody (82469-6-RR)

    WB analysis of mouse spleen using 82469-6-RR

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Bcl2 Recombinant antibody (82469-6-RR)

    WB analysis using 82469-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Bcl2 Recombinant antibody (82469-6-RR)

    FC experiment of NIH/3T3 using 82469-6-RR

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Recombinant antibody (82469-6-RR, Clone:240571H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82469-6-RR

    Affinity and Kinetic Characterization of 82469-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82469-6-RR against Mouse Bcl2 were performed. The affinity constant is 1.08 nM.

Tested Applications

Positive WB detected inmouse kidney tissue, mouse spleen tissue, mouse heart tissue
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82469-6-RR is Bcl2 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Bcl2 fusion protein Ag24260
Full Name B-cell leukemia/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocols

Product Specific Protocols
WB protocol for Bcl2 antibody 82469-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols