Cytokeratin 20 Recombinant antibody, PBS Only

Cytokeratin 20 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10

Cat no : 82428-1-PBS

Synonyms

KRT20, keratin 20, K20, Cytokeratin-20, CK-20

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Validation Data Gallery

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  WB analysis using 82428-1-RR (same clone as 82428-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 82428-1-RR (same clone as 82428-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:22000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 82428-1-RR (same clone as 82428-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 82428-1-RR (Cytokeratin 20 antibody) at dilution of 1:1100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using Cytokeratin 20 antibody (82428-1-RR, Clone: 2H10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 82428-1-RR (same clone as 82428-1-PBS)

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 20 (82428-1-RR, Clone:2H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82428-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82428-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82428-1-RR against Human Cytokeratin 20 were performed. The affinity constant is below 1 pM.

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Product Information

82428-1-PBS targets Cytokeratin 20 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cytokeratin 20 fusion protein Ag11308
• Full Name: keratin 20
• Calculated Molecular Weight: 424 aa, 48 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC031559
• Gene Symbol: Cytokeratin 20
• Gene ID (NCBI): 54474
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.