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EGLN3/PHD3 Recombinant antibody

EGLN3/PHD3 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1O19

Cat no : 82377-1-RR

Synonyms

Egl nine homolog 3, EGLN3, FLJ21620, HIF PH3, HIF prolyl hydroxylase 3, HIFPH3, HPH 1, HPH 3, PHD3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    WB analysis using 82377-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82377-1-RR (EGLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    IF Staining of HepG2 using 82377-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using EGLN3/PHD3 Recombinant antibody (82377-1-RR)

    IF Staining of U2OS using 82377-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using EGLN3/PHD3 antibody (82377-1-RR, Clone: 1O19 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IF detected inHepG2 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82377-1-RR targets EGLN3/PHD3 in WB, IF, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EGLN3/PHD3 fusion protein Ag13197
Full Name egl nine homolog 3 (C. elegans)
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27-30 kDa
GenBank Accession NumberBC010992
Gene Symbol EGLN3
Gene ID (NCBI) 112399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.

Protocols

Product Specific Protocols
WB protocol for EGLN3/PHD3 antibody 82377-1-RRDownload protocol
IF protocol for EGLN3/PHD3 antibody 82377-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols