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Phospho-CREB1 (Ser133) Recombinant antibody

Phospho-CREB1 (Ser133) Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5H5

Cat no : 81871-1-RR

Synonyms

CREB, CREB 1, CREB1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    WB analysis of HEK-293 using 81871-1-RR

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:20000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta-tubulin antibody as loading control.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    IHC staining of human prostate cancer using 81871-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Phospho-CREB1 (Ser133) Recombinant antibody (81871-1-RR)

    IHC staining of human prostate cancer using 81871-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81871-1-RR (Phospho-CREB1 (Ser133) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inCalyculin A treated HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81871-1-RR targets Phospho-CREB1 (Ser133) in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name cAMP responsive element binding protein 1
Calculated Molecular Weight 341 aa, 35 kDa
Observed Molecular Weight 35-46 kDa
GenBank Accession NumberBC010636
Gene Symbol CREB1
Gene ID (NCBI) 1385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. The antibody also detects the phosphorylated form of the CREB-related protein ATF1. (PMID: 30214393, PMID: 33917483, PMID: 32605164)

Protocols

Product Specific Protocols
WB protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RRDownload protocol
IHC protocol for Phospho-CREB1 (Ser133) antibody 81871-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols