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IL-6 Recombinant antibody, PBS Only (Capture)

IL-6 Recombinant Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

2E9

Cat no : 81077-1-PBS

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using IL-6 Recombinant antibody (81077-1-RR)

    WB analysis of HUVEC using 81077-1-RR (same clone as 81077-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81077-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-87 MG cells using IL-6 Recombinant antibody (81077-1-RR)

    WB analysis of U-87 MG using 81077-1-RR (same clone as 81077-1-PBS)

    Protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81077-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP80002-1

    Cytometric bead array standard curve of MP80002-1

    Cytometric bead array standard curve of MP80002-1, IL-6 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 81077-1-RR. Detection antibody: 68659-10-PBS. Standard:null. Range: 1.563-100 ng/mL.

  • Cytometric bead array standard curve of MP80002-1

    Cytometric bead array standard curve of MP80002-1

    Cytometric bead array standard curve of MP80002-1, IL-6 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 81077-1-PBS. Detection antibody: 68659-10-PBS. Standard:HZ-1019 . Range: 1.563-100 ng/mL.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81077-1-PBS targets IL-6 as part of a matched antibody pair:

MP80002-1: 81077-1-PBS capture and 68659-10-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-6 fusion protein Ag10565
Full Name interleukin 6 (interferon, beta 2)
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.