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  • Featured Product
  • KD/KO Validated

AKT1-Specific Recombinant antibody

AKT1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

5O9

Cat no : 80816-1-RR

Synonyms

AKT pan, AKT 1, AKT (pan), AKT, AKT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT1 Recombinant antibody (80816-1-RR)

    WB analysis using 80816-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AKT1 Recombinant antibody (80816-1-RR)

    WB analysis of MCF-7 using 80816-1-RR

    WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using AKT1 Recombinant antibody (80816-1-RR)

    IP experiment of HEK-293 using 80816-1-RR

    IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AKT1 Recombinant antibody (80816-1-RR)

    IHC staining of human ovary tumor using 80816-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AKT1 Recombinant antibody (80816-1-RR)

    IHC staining of human ovary tumor using 80816-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of Jurkat cells using AKT1-Specific Recombinant antibody (80816-1-RR)

    FC experiment of Jurkat using 80816-1-RR

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 80816-1-RR

    Affinity and Kinetic Characterization of 80816-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80816-1-RR against Human AKT1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells, HSC-T6 cells, PC-12 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

80816-1-RR targets AKT1-Specific in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AKT1-Specific fusion protein Ag0213
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.

Protocols

Product Specific Protocols
WB protocol for AKT1-Specific antibody 80816-1-RRDownload protocol
IHC protocol for AKT1-Specific antibody 80816-1-RRDownload protocol
IP protocol for AKT1-Specific antibody 80816-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int Immunopharmacol

Geniposide ameliorates psoriatic skin inflammation by inhibiting the TLR4/MyD88/NF-κB p65 signaling pathway and MMP9

Authors - Lijuan Liu
View Article