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Phospho-BCL2 (Ser70) Recombinant antibody

Phospho-BCL2 (Ser70) Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230061A8

Cat no : 80771-2-RR

Synonyms

BCL-2, BCL2, 230061A8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    WB analysis using 80771-2-RR

    Non-treated Jurkat cells, paclitaxel treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Phospho-BCL2 (Ser70) Recombinant antibody (80771-2-RR)

    IHC staining of human tonsillitis using 80771-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80771-2-RR (Phospho-BCL2 (Ser70) antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, paclitaxel treated Jurkat cells, Calyculin A treated Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80771-2-RR targets Phospho-BCL2 (Ser70) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name B-cell CLL/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359)

Protocols

Product Specific Protocols
WB protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RRDownload protocol
IHC protocol for Phospho-BCL2 (Ser70) antibody 80771-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols