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Phospho-STAT3 (Ser727) Recombinant antibody

Phospho-STAT3 (Ser727) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240778A12

Cat no : 80199-2-RR

Synonyms

p STAT3 (Ser727), p STAT3, p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Phospho-STAT3 (Ser727) Recombinant antibody (80199-2-RR)

    WB analysis of HeLa using 80199-2-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 80199-2-RR (Phospho-STAT3 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293T cells using Phospho-STAT3 (Ser727) Recombinant antibody (80199-2-RR)

    FC experiment of HEK-293T using 80199-2-RR

    1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.25 ug Stat3 Recombinant Antibody (80199-2-RR, Clone:240778A12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive WB detected inHeLa cells
Positive FC (Intra) detected inCalyculin A treated HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80199-2-RR targets Phospho-STAT3 (Ser727) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated Molecular Weight 770 aa, 88 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC000627
Gene Symbol STAT3
Gene ID (NCBI) 6774
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT3 (Ser727) antibody 80199-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols