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  • Western Blot (WB) analysis of various lysates using STAT2 Recombinant antibody (80061-5-RR)

    WB analysis using 80061-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80061-5-RR (STAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 80061-5-RR

    Affinity and Kinetic Characterization of 80061-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80061-5-RR against Human STAT2 were performed. The affinity constant is below 1 pM.

STAT2 Recombinant antibody

STAT2 {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 80061-5-RR
Clone No.242685A1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

STAT113, STAT 2, P113, ISGF3, ISGF 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80061-5-RR targets STAT2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STAT2 fusion protein Ag10168 Predict reactive species
Full Name signal transducer and activator of transcription 2, 113kDa
Calculated Molecular Weight 851 aa, 98 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC051284
Gene Symbol STAT2
Gene ID (NCBI) 6773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52630
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Protocols

Product Specific Protocols
WB protocol for STAT2 antibody 80061-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols