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NeutraKine®IL-12 p35 Monoclonal antibody

NeutraKine®IL-12 p35 Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

2G11G6

Cat no : 69029-1-Ig

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL-12 p35, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Validation Data Gallery

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  • Neutralization experiment of NeutraKine®IL-12 p35 using NeutraKine®IL-12 p35 Monoclonal antibody (69029-1-Ig)

    Neutralization experiment of NeutraKine®IL-12 p35 using 69029-1-Ig

    Recombinant human IL-12 (Cat.NO. HZ-1256) stimulates NK-92 cells produce IFN gamma in a dose dependent manner (blue curve, refer to bottom X-Left Y axis). The activity of human IL-12 (10 ng/mL HZ-1256) is neutralized by mouse anti-human IL-12 p35 monoclonal antibody 69029-1-Ig (red curve, refer to top X-right Y axis). The ND50 is typically 0.5-1 μg/mL. The control mouse anti-human IL-12 p35 monoclonal antibody 69529-1-Ig could recognize human IL-12, but could not neutralize human IL-12 at serial does at the range of 0.00122-20 μg/mL(green curve, refer to top X-right Y).

  • ELISA experiment of Recombinant protein using NeutraKine®IL-12 p35 Monoclonal antibody (69029-1-Ig)

    ELISA experiment of Recombinant protein using 69029-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer but not recognize IL-12 p40.

Neutralization

Product Information

69029-1-Ig targets NeutraKine®IL-12 p35 in ELISA, Neutralization applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen human HumankineIL-12 p35 protein HZ-1256
Full Name interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Gene Symbol IL-12A
Gene ID (NCBI) 3592
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein A purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

IL-12 p35 is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

This antibody can be used to neutralize the bioactivity of IL-12 p35.