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NeutraKine® IL-12/IL-23 p40 Monoclonal antibody

NeutraKine® IL-12/IL-23 p40 Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

2A9H6

Cat no : 69006-1-Ig

Synonyms

Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), IL 23, IL-12 subunit p40, IL-12/IL-23 p40, IL12B, IL-12B, IL-23 p40, IL23A, IL-23B, IL23P19, NeutraKine® IL-23 p40, SGRF

Validation Data Gallery

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  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces invitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL.

  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-23 (Cat.NO. HZ-1254) at 70 ng/well followed by blocking and adding serial diluted IL-23 antibody 69006-1-Ig and 69506-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • ELISA experiment of Recombinant protein using NeutraKine® IL-12/IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer) but not recognize IL-12/IL-23 p40.

Neutralization

Product Information

69006-1-Ig targets NeutraKine® IL-12/IL-23 p40 in ELISA, Neutralization applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen human Humankine IL-12/IL-23 p40 protein HZ-1254
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Calculated Molecular Weight 328 aa, 37 kDa
GenBank Accession NumberBC067498
Gene Symbol IL12B
Gene ID (NCBI) 51561,3593
RRIDAB_2882924
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

This antibody can be used to neutralize the bioactivity of IL-12/IL-23 p40.