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ARF6 Monoclonal antibody

ARF6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5H1

Cat no : 68924-1-Ig

Synonyms

EC:3.6.5.2, ADP-ribosylation factor 6, ADP ribosylation factor 6, 1H5H1

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using ARF6 Monoclonal antibody (68924-1-Ig)

    WB analysis of LNCaP using 68924-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ARF6 Monoclonal antibody (68924-1-Ig)

    WB analysis of MCF-7 using 68924-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ARF6 Monoclonal antibody (68924-1-Ig)

    WB analysis of HEK-293 using 68924-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using ARF6 Monoclonal antibody (68924-1-Ig)

    WB analysis of pig liver using 68924-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 68924-1-Ig (ARF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, MCF-7 cells, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68924-1-Ig targets ARF6 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARF6 fusion protein Ag12642
Full Name ADP-ribosylation factor 6
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC002952
Gene Symbol ARF6
Gene ID (NCBI) 382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

Protocols

Product Specific Protocols
WB protocol for ARF6 antibody 68924-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols