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S100A9 Monoclonal antibody

S100A9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E12C1

Cat no : 68900-1-Ig

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human saliva using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of human saliva using 68900-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-468 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of MDA-MB-468 using 68900-1-Ig

    MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1376 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of HT-1376 using 68900-1-Ig

    HT-1376 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SK-BR-3 cells using S100A9 Monoclonal antibody (68900-1-Ig)

    WB analysis of SK-BR-3 using 68900-1-Ig

    SK-BR-3 cells were subjected to SDS PAGE followed by western blot with 68900-1-Ig (S100A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman saliva, HT-1376 cells, MDA-MB-468 cells, SK-BR-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68900-1-Ig targets S100A9 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A9 fusion protein Ag27025
Full Name S100 calcium binding protein A9
Calculated Molecular Weight 13 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC047681
Gene Symbol S100A9
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.

Protocols

Product Specific Protocols
WB protocol for S100A9 antibody 68900-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols