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GDF-15 Monoclonal antibody, PBS Only (Capture/Detector)

GDF-15 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1H4

Cat no : 68768-1-PBS

Synonyms

GDF15, MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF 15

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GDF-15 Monoclonal antibody (68768-1-Ig)

    WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GDF-15 Monoclonal antibody (68768-1-Ig)

    WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

    Untreated and Brefeldin A treated LNCaP cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GDF-15 Monoclonal antibody (68768-1-Ig)

    WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Caki-1 cells using GDF-15 Monoclonal antibody (68768-1-Ig)

    WB analysis of Caki-1 using 68768-1-Ig (same clone as 68768-1-PBS)

    Caki-1 cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50133-8

    Sandwich ELISA standard curve of MP50133-8

    Sandwich ELISA standard curve of MP50133-8, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-5-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50133-9

    Sandwich ELISA standard curve of MP50133-9

    Sandwich ELISA standard curve of MP50133-9, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-6-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50133-1

    Sandwich ELISA standard curve of MP50133-1

    Sandwich ELISA standard curve of MP50133-1, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-3-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50133-2

    Sandwich ELISA standard curve of MP50133-2

    Sandwich ELISA standard curve of MP50133-2, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-4-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50133-4

    Sandwich ELISA standard curve of MP50133-4

    Sandwich ELISA standard curve of MP50133-4, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-7-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

  • Sandwich ELISA standard curve of MP50133-5

    Sandwich ELISA standard curve of MP50133-5

    Sandwich ELISA standard curve of MP50133-5, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-1-PBS. Detection antibody: HRP-conjugated 68768-2-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

Product Information

68768-1-PBS targets GDF-15 as part of a matched antibody pair:

MP50133-1: 68768-3-PBS capture and 68768-1-PBS detection (validated in Sandwich ELISA)

MP50133-2: 68768-4-PBS capture and 68768-1-PBS detection (validated in Sandwich ELISA)

MP50133-4: 68768-7-PBS capture and 68768-1-PBS detection (validated in Sandwich ELISA)

MP50133-5: 68768-1-PBS capture and 68768-2-PBS detection (validated in Sandwich ELISA)

MP50133-8: 68768-5-PBS capture and 68768-1-PBS detection (validated in Sandwich ELISA)

MP50133-9: 68768-6-PBS capture and 68768-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GDF-15 fusion protein Ag33750
Full Name growth differentiation factor 15
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.