• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GDF15 Monoclonal antibody

GDF15 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B1H4

Cat no : 68768-1-Ig

Synonyms

MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF-15, GDF 15

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GDF15 Monoclonal antibody (68768-1-Ig)

    WB analysis using 68768-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GDF15 Monoclonal antibody (68768-1-Ig)

    WB analysis using 68768-1-Ig

    Untreated and Brefeldin A treated LNCaP cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GDF15 Monoclonal antibody (68768-1-Ig)

    WB analysis using 68768-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caki-1 cells using GDF15 Monoclonal antibody (68768-1-Ig)

    WB analysis of Caki-1 using 68768-1-Ig

    Caki-1 cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, Caki-1 cells, HepG2 cells, LNCaP cells, HT-29 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68768-1-Ig targets GDF15 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GDF15 fusion protein Ag33750
Full Name growth differentiation factor 15
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC008962
Gene Symbol GDF15
Gene ID (NCBI) 9518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Protocols

Product Specific Protocols
WB protocol for GDF15 antibody 68768-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols