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ATP1A1 Monoclonal antibody

ATP1A1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E9B7

Cat no : 68735-2-Ig

Synonyms

ATP1A1, Sodium pump subunit alpha 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP1A1 Monoclonal antibody (68735-2-Ig)

    WB analysis using 68735-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68735-2-Ig (ATP1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP1A1 Monoclonal antibody (68735-2-Ig)

    IHC staining of mouse kidney using 68735-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP1A1 Monoclonal antibody (68735-2-Ig)

    IHC staining of mouse kidney using 68735-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68735-2-Ig (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ATP1A1 Monoclonal antibody (68735-2-Ig)

    IF Staining of HEK-293 using 68735-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATP1A1 antibody (68735-2-Ig, Clone: 1E9B7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Tested Applications

Positive WB detected inunboiled A431 cells, unboiled HepG2 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68735-2-Ig targets ATP1A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATP1A1 fusion protein Ag5990
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for ATP1A1 antibody 68735-2-IgDownload protocol
IHC protocol for ATP1A1 antibody 68735-2-IgDownload protocol
IF protocol for ATP1A1 antibody 68735-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols