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SDCCAG1 Monoclonal antibody

SDCCAG1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C8A1

Cat no : 68708-1-Ig

Synonyms

Ribosome quality control complex subunit NEMF, NY CO 1, Nuclear export mediator factor, NEMF, Homo sapiens (Human)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDCCAG1 Monoclonal antibody (68708-1-Ig)

    WB analysis using 68708-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68708-1-Ig (SDCCAG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68708-1-Ig targets SDCCAG1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SDCCAG1 fusion protein Ag2467
Full Name serologically defined colon cancer antigen 1
Calculated Molecular Weight 1076 aa, 123 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC020794
Gene Symbol SDCCAG1
Gene ID (NCBI) 9147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).

Protocols

Product Specific Protocols
WB protocol for SDCCAG1 antibody 68708-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols