Filter: AllSandwich ELISASample test

Sandwich ELISA standard curve of MP50085-1
Sandwich ELISA standard curve of MP50085-1, ICAM-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65075-1-PBS. Detection antibody: 68693-1-PBS. Standard: Eg0570. Range: 62.5-4000 pg/mL.
Sample test of MP50085-1
Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean ICAM-1/CD54 concentration was determined to be 2,139.3 pg/mL in unstimulated PBMC supernatant, 30,678.8 pg/mL in PHA stimulated PBMC supernatant.
Sample test of MP50085-1
Saliva of seven individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 5,597.4 pg/mL with a range of 2,577.6 - 11,698.1 pg/mL.
Sample test of MP50085-1
Urine of seven individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 1,031.9 pg/mL with a range of 49.7 - 3,299.7 pg/mL.
Sample test of MP50085-1
Serum of sixteen individual healthy human donors was measured. The human ICAM-1/CD54 concentration of detected samples was determined to be 260.08 ng/mL with a range of 142.73 - 650.42 ng/mL.

ICAM-1/CD54 Monoclonal antibody, PBS Only (Detector)

ICAM-1/CD54 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68693-1-PBS

Clone No.2C9G8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD54, ICAM-1, BB2, ICAM 1, ICAM1

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

68693-1-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP50085-1: 65075-1-PBS capture and 68693-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Eg0570 Product name: Recombinant Human ICAM-1/CD54 protein (His Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 28-480 aa of BC015969 Sequence: QTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLTVVLLRGEKELKREPAVGEPAEVTTTVLVRRDHHGANFSCRTELDLRPQGLELFENTSAPYQLQTFVLPATPPQLVSPRVLEVDTQGTVVCSLDGLFPVSEAQVHLALGDQRLNPTVTYGNDSFSAKASVSVTAEDEGTQRLTCAVILGNQSQETLQTVTIYSFPAPNVILTKPEVSEGTEVTVKCEAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLIHKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCLKDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSPRYE Predict reactive species
Full Name	intercellular adhesion molecule 1
Calculated Molecular Weight	90 kDa
GenBank Accession Number	BC015969
Gene Symbol	ICAM-1
Gene ID (NCBI)	3383
ENSEMBL Gene ID	ENSG00000090339
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P05362
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.