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Anti-Human ICAM-1/CD54 (15.2), PBS Only

ICAM-1/CD54 Monoclonal Antibody for FC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

15.2

Cat no : 65075-1-PBS

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1

    Sandwich ELISA standard curve of MP50085-1, ICAM-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 65075-1-PBS. Detection antibody: 68693-1-PBS. Standard: Eg0570. Range: 62.5-4000 pg/mL.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) (65075-1-Ig)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ICAM-1/CD54 (15.2) (65075-1-Ig)

    FC experiment of human PBMCs using 65075-1-Ig (same clone as 65075-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD54 (ICAM-1) (65075-1-Ig, Clone:15.2) and APC-Donkey anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65075-1-PBS in a different storage buffer formulation.

Product Information

65075-1-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP50085-1: 65075-1-PBS capture and 68693-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes
Full Name intercellular adhesion molecule 1
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).