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CD86 Monoclonal antibody, PBS Only (Detector)

CD86 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6H7

Cat no : 68674-2-PBS

Synonyms

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD86 Monoclonal antibody (68674-2-Ig)

    WB analysis using 68674-2-Ig (same clone as 68674-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68674-2-Ig (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68674-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD86 Monoclonal antibody (68674-2-Ig)

    WB analysis using 68674-2-Ig (same clone as 68674-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68674-2-Ig (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68674-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50056-1

    Sandwich ELISA standard curve of MP50056-1

    Sandwich ELISA standard curve of MP50056-1, CD86 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68674-1-PBS. Detection antibody: 68674-2-PBS. Standard: Eg0059. Range: 78.1-5000 pg/mL.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68674-2-PBS targets CD86 as part of a matched antibody pair:

MP50056-1: 68674-1-PBS capture and 68674-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein
Full Name CD86 molecule
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberNM_175862
Gene Symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.