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RS1 Monoclonal antibody

RS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A2D4

Cat no : 68654-1-Ig

Synonyms

Retinoschisin, retinoschisin 1, RS, RS1, XLRS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RS1 Monoclonal antibody (68654-1-Ig)

    WB analysis using 68654-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68654-1-Ig (RS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse retina tissue using RS1 Monoclonal antibody (68654-1-Ig)

    WB analysis of mouse retina using 68654-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68654-1-Ig (RS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inrabbit retina tissue, mouse retina tissue, rat retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68654-1-Ig targets RS1 in WB, ELISA applications and shows reactivity with Human, rat, mouse, rabbit samples.

Tested Reactivity Human, rat, mouse, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RS1 fusion protein Ag18885
Full Name retinoschisin 1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC141638
Gene Symbol RS1
Gene ID (NCBI) 6247
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RS1 is also named as XLRS1. RS1 can bind negatively charged membrane lipids, such as phosphatidylserine and phosphoinositides. RS1 is required for normal structure and function of the retina (PMID:19093009). It may play a role in cell-cell adhesion processes in the retina (PMID:27114531). RS1 is high expression in the retina (at protein level) (PMID:10915776, PMID:9326935). It is detected in the inner segment of the photoreceptors, the inner nuclear layer, the inner plexiform layer and the ganglion cell layer. At the macula, it is expressed in both the outer and inner nuclear layers and in the inner plexiform layer (PMID:10915776, PMID:10915776). And RS1 is undetectable in the inner plexiform layers and the inner nuclear layer (PMID:10915776)

Protocols

Product Specific Protocols
WB protocol for RS1 antibody 68654-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols