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FBXO22 Monoclonal antibody

FBXO22 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B8A12

Cat no : 68650-1-Ig

Synonyms

F box only protein 22, F box protein 22, F box protein FBX22p44, FBX22, FBXO22, FISTC1, FLJ13986

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO22 Monoclonal antibody (68650-1-Ig)

    WB analysis using 68650-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68650-1-Ig (FBXO22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68650-1-Ig targets FBXO22 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FBXO22 fusion protein Ag5294
Full Name F-box protein 22
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC039024
Gene Symbol FBXO22
Gene ID (NCBI) 26263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO22, also named as FBX22, is a substrate-recognition of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. FBXO22 is a key regulator of senescence induction through ubiquitylation of p53 for degradation. FBXO22 also acts as a molecular switch for the antagonistic and agonistic actions of selective estrogen receptor modulators (SERM) and determines the sensitivity of breast cancer to SERM by ubiquitylating KDM4B complexed with unliganded or SERMs‐bound estrogen receptor (ER). (PMID: 32536008, PMID: 32249768)

Protocols

Product Specific Protocols
WB protocol for FBXO22 antibody 68650-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols