Filter: AllWBIF/ICCCytometric bead array

WB analysis using 68642-1-Ig (same clone as 68642-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.
WB analysis of LNCaP using 68642-1-Ig (same clone as 68642-1-PBS)
LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51262-1
Cytometric bead array standard curve of MP51262-1, COG1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68642-1-PBS. Detection antibody: 68642-2-PBS. Standard:Ag33924. Range: 3.125-200 ng/mL.

COG1 Monoclonal antibody, PBS Only (Capture)

COG1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68642-1-PBS

Clone No.2D8D11

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68642-1-PBS targets COG1 as part of a matched antibody pair:

MP51262-1: 68642-1-PBS capture and 68642-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	COG1 fusion protein Ag33924 Predict reactive species
Full Name	component of oligomeric golgi complex 1
Calculated Molecular Weight	109 kDa
Observed Molecular Weight	100-109 kDa
GenBank Accession Number	BC021985
Gene Symbol	COG1
Gene ID (NCBI)	9382
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q8WTW3
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).