COG1 Monoclonal antibody

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  WB analysis using 68642-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 68642-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 68642-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of U2OS using 68642-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

COG1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68642-1-Ig

Clone No.2D8D11

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

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Tested Applications

• Positive WB detected in: HepG2 cells, LNCaP cells, Jurkat cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68642-1-Ig targets COG1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.

• Tested Reactivity: human, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: COG1 fusion protein Ag33924
• Full Name: component of oligomeric golgi complex 1
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 100-109 kDa
• GenBank Accession Number: BC021985
• Gene Symbol: COG1
• Gene ID (NCBI): 9382
• RRID: AB_3670386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8WTW3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).