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TOX2 Monoclonal antibody

TOX2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G12A7

Cat no : 68590-1-Ig

Synonyms

C20orf100, dJ1108D11.2, dJ495O3.1, GCX 1, GCX1, TOX2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOX2 Monoclonal antibody (68590-1-Ig)

    WB analysis using 68590-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68590-1-Ig (TOX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using TOX2 Monoclonal antibody (68590-1-Ig)

    WB analysis using 68590-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68590-1-Ig (TOX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inSH-SY5Y cells, A549 cells, HeLa cells, HT-1080 cells, HEK-293 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68590-1-Ig targets TOX2 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TOX2 fusion protein Ag31563
Full Name TOX high mobility group box family member 2
Calculated Molecular Weight52KD
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_001098797
Gene Symbol TOX2
Gene ID (NCBI) 84969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

Protocols

Product Specific Protocols
WB protocol for TOX2 antibody 68590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols