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SLC1A5/ASCT2 Monoclonal antibody

SLC1A5/ASCT2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C4E1

Cat no : 68540-1-Ig

Synonyms

SLC1A5, Baboon M7 virus receptor, ATBO, ATB(0), ASCT2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    WB analysis using 68540-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68540-1-Ig (SLC1A5/ASCT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    IHC staining of human stomach cancer using 68540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    IHC staining of human stomach cancer using 68540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    IF Staining of HEK-293 using 68540-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (68540-1-Ig, Clone: 1C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HeLa cells using SLC1A5/ASCT2 Monoclonal antibody (68540-1-Ig)

    FC experiment of HeLa using 68540-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (68540-1-Ig, Clone:1C4E1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA431 cells, PNGase F-treated A431 cells, A549 cells, PNGase F-treated A549 cells, HT-29 cells, PNGase F-treated HT-29 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68540-1-Ig targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SLC1A5/ASCT2 fusion protein Ag16837
Full Name solute carrier family 1 (neutral amino acid transporter), member 5
Calculated Molecular Weight 541 aa, 57 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000062
Gene Symbol SLC1A5/ASCT2
Gene ID (NCBI) 6510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).

Protocols

Product Specific Protocols
WB protocol for SLC1A5/ASCT2 antibody 68540-1-IgDownload protocol
IHC protocol for SLC1A5/ASCT2 antibody 68540-1-IgDownload protocol
IF protocol for SLC1A5/ASCT2 antibody 68540-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols