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EXOSC7 Monoclonal antibody

EXOSC7 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A4C6

Cat no : 68456-1-Ig

Synonyms

KIAA0116, hRrp42p, Exosome component 7, Exosome complex component RRP42, EAP1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EXOSC7 Monoclonal antibody (68456-1-Ig)

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EXOSC7 Monoclonal antibody (68456-1-Ig)

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using EXOSC7 Monoclonal antibody (68456-1-Ig)

    WB analysis using 68456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68456-1-Ig (EXOSC7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSaos-2 cells, HSC-T6 cells, PANC-1 cells, NIH/3T3 cells, 4TA cells, HT-29 cells, LNCaP cells, MDA-MB-231 cells, IMR-32 cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, pig brain tissue, Caco-2 cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68456-1-Ig targets EXOSC7 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EXOSC7 fusion protein Ag18322
Full Name exosome component 7
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC012831
Gene Symbol EXOSC7
Gene ID (NCBI) 23016
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA.

Protocols

Product Specific Protocols
WB protocol for EXOSC7 antibody 68456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols