Filter: AllWBSandwich ELISASample testIF/ICC

WB analysis using 68383-1-Ig (same clone as 68383-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.
WB analysis of HUVEC using 68383-1-Ig (same clone as 68383-1-PBS)
HUVEC cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 68383-1-Ig (same clone as 68383-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.
WB analysis of pig kidney using 68383-1-Ig (same clone as 68383-1-PBS)
pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.
WB analysis of rabbit kidney using 68383-1-Ig (same clone as 68383-1-PBS)
rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP50051-1
Sandwich ELISA standard curve of MP50051-1, DPP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68383-1-PBS. Detection antibody: 68383-2-PBS. Standard: Eg0157. Range: 0.313-20 ng/mL.
Sample test of MP50051-1
Urine of eight individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 33.23 ng/mL with a range of 9.07-105.84 ng/mL.
Sample test of MP50051-1
Saliva of eight individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 33.57 ng/mL with a range of 21.27-77.69 ng/mL.
Sample test of MP50051-1
Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean DPP4/CD26 concentration was determined to be 0.17 ng/mL in unstimulated PBMC supernatant, 1.84 ng/mL in PHA stimulated PBMC supernatant.
Sample test of MP50051-1
Serum of sixteen individual healthy human donors was measured. The DPP4/CD26 concentration of detected samples was determined to be 475.10 ng/mL with a range of 189.59-990.11 ng/mL.
IF Staining of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

DPP4/CD26 Monoclonal antibody, PBS Only (Capture)

DPP4/CD26 Monoclonal Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68383-1-PBS

Clone No.2G6D11

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD26, 2G6D11, ADABP, ADCP 2, ADCP2

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68383-1-PBS targets DPP4/CD26 as part of a matched antibody pair:

MP50051-1: 68383-1-PBS capture and 68383-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Eg0157 Product name: Recombinant Human DPP4/CD26 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 34-766 aa of BC013329 Sequence: DATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP Predict reactive species
Full Name	dipeptidyl-peptidase 4
Calculated Molecular Weight	88 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC013329
Gene Symbol	DPP4
Gene ID (NCBI)	1803
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P27487
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).