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POLR3D Monoclonal antibody

POLR3D Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rabbit

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F5G4

Cat no : 68263-1-Ig

Synonyms

RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T, BN51

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using POLR3D Monoclonal antibody (68263-1-Ig)

    WB analysis of HEK-293 using 68263-1-Ig

    WB result of POLR3D antibody (68263-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR3D transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using POLR3D Monoclonal antibody (68263-1-Ig)

    WB analysis using 68263-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rabbit brain tissue using POLR3D Monoclonal antibody (68263-1-Ig)

    WB analysis of rabbit brain using 68263-1-Ig

    rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using POLR3D Monoclonal antibody (68263-1-Ig)

    WB analysis of K-562 using 68263-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using POLR3D Monoclonal antibody (68263-1-Ig)

    FC experiment of HeLa using 68263-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug POLR3D Monoclonal antibody (68263-1-Ig, Clone:2F5G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inLNCaP cells, rabbit brain tissue, K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68263-1-Ig targets POLR3D in WB, FC (Intra), ELISA applications and shows reactivity with human, rabbit samples.

Tested Reactivity human, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen POLR3D fusion protein Ag7646
Full Name polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC004484
Gene Symbol POLR3D
Gene ID (NCBI) 661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.

Protocols

Product Specific Protocols
WB protocol for POLR3D antibody 68263-1-IgDownload protocol
FC protocol for POLR3D antibody 68263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols