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CNTN1 Monoclonal antibody

CNTN1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C1D4

Cat no : 68252-1-Ig

Synonyms

CNTN1, contactin 1, F3, Glycoprotein gp135, GP135, Neural cell surface protein F3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNTN1 Monoclonal antibody (68252-1-Ig)

    WB analysis using 68252-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68252-1-Ig (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CNTN1 Monoclonal antibody (68252-1-Ig)

    WB analysis using 68252-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68252-1-Ig (CNTN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, pig cerebellum tissue, pig brain tissue, rabbit brain tissue, rabbit cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68252-1-Ig targets CNTN1 in WB, ELISA applications and shows reactivity with Human, pig, rabbit samples.

Tested Reactivity Human, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNTN1 fusion protein Ag5030
Full Name contactin 1
Calculated Molecular Weight 1018 aa, 113 kDa
Observed Molecular Weight130-135 kDa
GenBank Accession NumberBC036569
Gene Symbol CNTN1
Gene ID (NCBI) 1272
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Contactin-1 (CNTN1), also known as Glycoprotein gp135 or Neural cell surface protein F3, is a neuronal cell adhesion molecule of the immunoglobulin superfamily (PMID: 3049624). It consists of six Ig domains, four fibronectin-like motifs, and a glycosylphosphatidylinositol (GPI)-moiety. Expressed by the central and peripheral neurons, CNTN1 contributes to the formation and function of neuronal connections. It plays a role in axon extension and formation of septate-like junctions between axons and myelinating glial cells (PMID: 23724143). In addition, acting as a ligand for Notch, CNTN1 initiates a NOTCH/DTX1 signaling pathway that promotes oligodendrocyte maturation and myelination (PMID: 14567914).

Protocols

Product Specific Protocols
WB protocol for CNTN1 antibody 68252-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols