Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68251-1-Ig targets TPMT in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TPMT fusion protein Ag28693 Predict reactive species |
| Full Name | thiopurine S-methyltransferase |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC005339 |
| Gene Symbol | TPMT |
| Gene ID (NCBI) | 7172 |
| RRID | AB_2935336 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51580 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TPMT antibody 68251-1-Ig | Download protocol |
| IF protocol for TPMT antibody 68251-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
