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TPMT Monoclonal antibody

TPMT Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D4B3

Cat no : 68251-1-Ig

Synonyms

Thiopurine methyltransferase, thiopurine S methyltransferase, TPMT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPMT Monoclonal antibody (68251-1-Ig)

    WB analysis using 68251-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68251-1-Ig (TPMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPMT Monoclonal antibody (68251-1-Ig)

    IF Staining of HepG2 using 68251-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TPMT antibody (68251-1-Ig, Clone: 1D4B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, k-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68251-1-Ig targets TPMT in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TPMT fusion protein Ag28693
Full Name thiopurine S-methyltransferase
Calculated Molecular Weight 28 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC005339
Gene Symbol TPMT
Gene ID (NCBI) 7172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.

Protocols

Product Specific Protocols
WB protocol for TPMT antibody 68251-1-IgDownload protocol
IF protocol for TPMT antibody 68251-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols